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KMID : 1138520020050020052
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Journal of Pharmacopuncture
2002 Volume.5 No. 2 p.52 ~ p.62
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The effects of Bee Venom on NO, $H_2O_2$ in Raw 264.7 cells and IL-1 in D10S cells
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Song Jeong-Yeol
Lee Seong-No
Jo Hyun-Chol
Kim Kee-Hyun
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Abstract
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Objectives : The purpose of this study was to investigate the effects of Bee Venom on NO, $H_2O_2$ expression induced by LPS in Raw 264.7 cells as a murine marcrophage cell line and on IL-1 expression induced by LPS in D10S cells.
Methods : The expression of NO was measured by MTT Assay and IL-1 by MTS Assay. The expression of $H_2O_2$ was measured as ROS level within the cell using by FACS analysis. The non-toxic concentration(from $0.1\;{\mu}g/ml\;to\;5\;{\mu}g/ml$) of Bee Venom was determined by MTT Assay.
Results : 1. Bee Venom inhibited the NO expression. The effective concentration of Bee Venom was $5\;{\mu}g/ml$ after 3 hours, 1 and $5\;{\mu}g/ml$ after 1 day and 2 days. The all concentration of Bee Venom inhibited the NO expression after 6, 12 hours and 3 days. 2. Bee Venom inhibited the $H_2O_2$ expression in a dose-dependent manner compared to the control. 3. Bee Venom could not significantly inhibit the IL-1 expression.
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KEYWORD
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Bee Venom, NO, $H_2O_2$, IL-1, Raw 264.7 cell, D10S cell
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